• Western blot analysis of extracts of various cell lines, using Acetyl-p53-K370 antibody (STJ11101783) at 1:1000 dilution. HeLa cells and NIH/3T3 cells were treated by TSA (1 uM) at 37 °C for 18 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 1s.
  • Western blot analysis of extracts of C6 cells, using Acetyl-p53-K370 antibody (STJ11101783) at 1:1000 dilution. C6 cells were treated by TSA (1 uM) at 37 °C for 18 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Acetyl-TP53-K370 antibody [ARC2368] (STJ11101783)


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Host: Rabbit
Applications: WB
Reactivity: Human, Mouse, Rat
Short Description: Rabbit monoclonal antibody anti-Acetyl-p53--K370 is suitable for use in Western Blot.
Clonality: Monoclonal
Clone ID: ARC2368
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS containing 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store in a freezer at-20°C and avoid freeze-thaw cycles.
Gene Symbol: TP53
Gene ID: 7157
Uniprot ID: P53_HUMAN
Immunogen: A synthetic acetylated peptide around K370 of human p53
Tissue Specificity Ubiquitous. Isoforms are expressed in a wide range of normal tissues but in a tissue-dependent manner. Isoform 2 is expressed in most normal tissues but is not detected in brain, lung, prostate, muscle, fetal brain, spinal cord and fetal liver. Isoform 3 is expressed in most normal tissues but is not detected in lung, spleen, testis, fetal brain, spinal cord and fetal liver. Isoform 7 is expressed in most normal tissues but is not detected in prostate, uterus, skeletal muscle and breast. Isoform 8 is detected only in colon, bone marrow, testis, fetal brain and intestine. Isoform 9 is expressed in most normal tissues but is not detected in brain, heart, lung, fetal liver, salivary gland, breast or intestine.
Post Translational Modifications Acetylated. Acetylation of Lys-382 by CREBBP enhances transcriptional activity. Deacetylation of Lys-382 by SIRT1 impairs its ability to induce proapoptotic program and modulate cell senescence. Deacetylation by SIRT2 impairs its ability to induce transcription activation in a AKT-dependent manner. Phosphorylation on Ser residues mediates transcriptional activation. Phosphorylated by HIPK1. Phosphorylation at Ser-9 by HIPK4 increases repression activity on BIRC5 promoter. Phosphorylated on Thr-18 by VRK1. Phosphorylated on Ser-20 by CHEK2 in response to DNA damage, which prevents ubiquitination by MDM2. Phosphorylated on Ser-20 by PLK3 in response to reactive oxygen species (ROS), promoting p53/TP53-mediated apoptosis. Phosphorylated on Thr-55 by TAF1, which promotes MDM2-mediated degradation. Phosphorylated on Ser-33 by CDK7 in a CAK complex in response to DNA damage. Phosphorylated on Ser-46 by HIPK2 upon UV irradiation. Phosphorylation on Ser-46 is required for acetylation by CREBBP. Phosphorylated on Ser-392 following UV but not gamma irradiation. Phosphorylated by NUAK1 at Ser-15 and Ser-392.was initially thought to be mediated by STK11/LKB1 but it was later shown that it is indirect and that STK11/LKB1-dependent phosphorylation is probably mediated by downstream NUAK1. It is unclear whether AMP directly mediates phosphorylation at Ser-15. Phosphorylated on Thr-18 by isoform 1 and isoform 2 of VRK2. Phosphorylation on Thr-18 by isoform 2 of VRK2 results in a reduction in ubiquitination by MDM2 and an increase in acetylation by EP300. Stabilized by CDK5-mediated phosphorylation in response to genotoxic and oxidative stresses at Ser-15, Ser-33 and Ser-46, leading to accumulation of p53/TP53, particularly in the nucleus, thus inducing the transactivation of p53/TP53 target genes. Phosphorylated by DYRK2 at Ser-46 in response to genotoxic stress. Phosphorylated at Ser-315 and Ser-392 by CDK2 in response to DNA-damage. Phosphorylation at Ser-15 is required for interaction with DDX3X and gamma-tubulin. Dephosphorylated by PP2A-PPP2R5C holoenzyme at Thr-55. SV40 small T antigen inhibits the dephosphorylation by the AC form of PP2A. May be O-glycosylated in the C-terminal basic region. Studied in EB-1 cell line. Ubiquitinated by MDM2 and SYVN1, which leads to proteasomal degradation. Ubiquitinated by RFWD3, which works in cooperation with MDM2 and may catalyze the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. Ubiquitinated by MKRN1 at Lys-291 and Lys-292, which leads to proteasomal degradation. Deubiquitinated by USP10, leading to its stabilization. Ubiquitinated by TRIM24, RFFL, RNF34 and RNF125, which leads to proteasomal degradation. Ubiquitination by TOPORS induces degradation. Deubiquitination by USP7, leading to stabilization. Isoform 4 is monoubiquitinated in an MDM2-independent manner. Ubiquitinated by COP1, which leads to proteasomal degradation. Ubiquitination and subsequent proteasomal degradation is negatively regulated by CCAR2. Polyubiquitinated by C10orf90/FATS, polyubiquitination is 'Lys-48'-linkage independent and non-proteolytic, leading to TP53 stabilization. Polyubiquitinated by MUL1 at Lys-24 which leads to proteasomal degradation. Monomethylated at Lys-372 by SETD7, leading to stabilization and increased transcriptional activation. Monomethylated at Lys-370 by SMYD2, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity. Lys-372 monomethylation prevents interaction with SMYD2 and subsequent monomethylation at Lys-370. Dimethylated at Lys-373 by EHMT1 and EHMT2. Monomethylated at Lys-382 by KMT5A, promoting interaction with L3MBTL1 and leading to repress transcriptional activity. Dimethylation at Lys-370 and Lys-382 diminishes p53 ubiquitination, through stabilizing association with the methyl reader PHF20. Demethylation of dimethylated Lys-370 by KDM1A prevents interaction with TP53BP1 and represses TP53-mediated transcriptional activation. Monomethylated at Arg-333 and dimethylated at Arg-335 and Arg-337 by PRMT5.methylation is increased after DNA damage and might possibly affect TP53 target gene specificity. Sumoylated with SUMO1. Sumoylated at Lys-386 by UBC9.
Function Acts as a tumor suppressor in many tumor types.induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Its pro-apoptotic activity is activated via its interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2. However, this activity is inhibited when the interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 is displaced by PPP1R13L/iASPP. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis.the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seems to have an effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2.
Protein Name Cellular Tumor Antigen P53
Antigen Ny-Co-13
Phosphoprotein P53
Tumor Suppressor P53
Database Links Reactome: R-HSA-111448
Reactome: R-HSA-139915
Reactome: R-HSA-1912408
Reactome: R-HSA-2559580
Reactome: R-HSA-2559584
Reactome: R-HSA-2559585
Reactome: R-HSA-2559586
Reactome: R-HSA-3232118
Reactome: R-HSA-349425
Reactome: R-HSA-390471
Reactome: R-HSA-5620971
Reactome: R-HSA-5628897
Reactome: R-HSA-5689880
Reactome: R-HSA-5689896
Reactome: R-HSA-5693565
Reactome: R-HSA-6785807
Reactome: R-HSA-6796648
Reactome: R-HSA-6803204
Reactome: R-HSA-6803205
Reactome: R-HSA-6803207
Reactome: R-HSA-6803211
Reactome: R-HSA-6804114
Reactome: R-HSA-6804115
Reactome: R-HSA-6804116
Reactome: R-HSA-6804754
Reactome: R-HSA-6804756
Reactome: R-HSA-6804757
Reactome: R-HSA-6804758
Reactome: R-HSA-6804759
Reactome: R-HSA-6804760
Reactome: R-HSA-6811555
Reactome: R-HSA-69473
Reactome: R-HSA-69481
Reactome: R-HSA-69541
Reactome: R-HSA-69895
Reactome: R-HSA-8852276
Reactome: R-HSA-8853884
Reactome: R-HSA-8941855
Reactome: R-HSA-8943724
Reactome: R-HSA-983231
Cellular Localisation Cytoplasm
Pml Body
Endoplasmic Reticulum
Mitochondrion Matrix
Microtubule Organizing Center
Recruited Into Pml Bodies Together With Chek2
Translocates To Mitochondria Upon Oxidative Stress
Translocates To Mitochondria In Response To Mitomycin C Treatment
Isoform 1: Nucleus
Predominantly Nuclear But Localizes To The Cytoplasm When Expressed With Isoform 4
Isoform 2: Nucleus
Localized Mainly In The Nucleus With Minor Staining In The Cytoplasm
Isoform 3: Nucleus
Localized In The Nucleus In Most Cells But Found In The Cytoplasm In Some Cells
Isoform 4: Nucleus
Predominantly Nuclear But Translocates To The Cytoplasm Following Cell Stress
Isoform 7: Nucleus
Isoform 8: Nucleus
Localized In Both Nucleus And Cytoplasm In Most Cells
In Some Cells
Forms Foci In The Nucleus That Are Different From Nucleoli
Isoform 9: Cytoplasm
Alternative Antibody Names Anti-Cellular Tumor Antigen P53 antibody
Anti-Antigen Ny-Co-13 antibody
Anti-Phosphoprotein P53 antibody
Anti-Tumor Suppressor P53 antibody
Anti-TP53 antibody
Anti-P53 antibody

Information sourced from Uniprot.org

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