• Western blot analysis of 1, hela 2, mouse-brain cells using primary antibody diluted at 1:1000 (4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800 ( diluted at 1:5000, 25°C, 1 hour)

Anti-Acetyl-E2F1-K117 antibody (STJ98838)

SKU:
STJ98838

Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Acetyl-Transcription factor E2F1-K117 is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: E2F1
Gene ID: 1869
Uniprot ID: E2F1_HUMAN
Specificity: This antibody detects endogenous levels of E2F-1 at Human:K117;Mouse:K112;Rat:K115, It doesn NA t reacte with total protein.
Immunogen: Synthesized Acetyl peptide derived from the human E2F-1. at the amino acid range K117
Function Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTCCGCGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters. Directly activates transcription of PEG10. Positively regulates transcription of RRP1B.
Protein Name Transcription Factor E2f1
E2f-1
Pbr3
Retinoblastoma-Associated Protein 1
Rbap-1
Retinoblastoma-Binding Protein 3
Rbbp-3
Prb-Binding Protein E2f-1
Database Links Reactome: R-HSA-111448
Reactome: R-HSA-113501
Reactome: R-HSA-1362277
Reactome: R-HSA-1362300
Reactome: R-HSA-139915
Reactome: R-HSA-1912408
Reactome: R-HSA-2559580
Reactome: R-HSA-2559585
Reactome: R-HSA-6804116
Reactome: R-HSA-68911
Reactome: R-HSA-69202
Reactome: R-HSA-69205
Reactome: R-HSA-69231
Reactome: R-HSA-69656
Reactome: R-HSA-8953750
Reactome: R-HSA-9616222
Reactome: R-HSA-9661069
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcription Factor E2f1 antibody
Anti-E2f-1 antibody
Anti-Pbr3 antibody
Anti-Retinoblastoma-Associated Protein 1 antibody
Anti-Rbap-1 antibody
Anti-Retinoblastoma-Binding Protein 3 antibody
Anti-Rbbp-3 antibody
Anti-Prb-Binding Protein E2f-1 antibody
Anti-E2F1 antibody
Anti-RBBP3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance