• Western blot analysis of extracts from Hela cells, untreated (-) or treated, 1:5000.. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Acetyl-H2AX-Lys5 antibody (STJ97189)


Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human, Mouse, Rat
Short Description: Rabbit polyclonal antibody anti-Acetyl-Histone H2ax-Lys5 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS, pH 7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol.
Purification: The antibody was affinity-purified from rabbit anti-serum by affinity-chromatography.
Dilution Range: WB 1:1000-2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: H2AX
Gene ID: 3014
Uniprot ID: H2AX_HUMAN
Specificity: Acetyl-H2AX-Lys5 polyclonal antibody (Histone H2ax) binds to endogenous Histone H2ax.
Immunogen: Synthetic peptide of Histone H2A.X (Acetyl Lys5)
Function Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
Protein Name Histone H2ax
Histone H2a.x
Database Links Reactome: R-HSA-110328
Reactome: R-HSA-110329
Reactome: R-HSA-110330
Reactome: R-HSA-110331
Reactome: R-HSA-1221632
Reactome: R-HSA-171306
Reactome: R-HSA-1912408
Reactome: R-HSA-201722
Reactome: R-HSA-212300
Reactome: R-HSA-2299718
Reactome: R-HSA-2559580
Reactome: R-HSA-2559582
Reactome: R-HSA-2559586
Reactome: R-HSA-3214858
Reactome: R-HSA-427359
Reactome: R-HSA-427389
Reactome: R-HSA-427413
Reactome: R-HSA-5250924
Reactome: R-HSA-5334118
Reactome: R-HSA-5578749
Reactome: R-HSA-5617472
Reactome: R-HSA-5625886
Reactome: R-HSA-5693565
Reactome: R-HSA-5693571
Reactome: R-HSA-5693607
Reactome: R-HSA-606279
Reactome: R-HSA-69473
Reactome: R-HSA-73728
Reactome: R-HSA-73772
Reactome: R-HSA-8936459
Reactome: R-HSA-8939236
Reactome: R-HSA-9018519
Reactome: R-HSA-912446
Reactome: R-HSA-9616222
Reactome: R-HSA-9670095
Reactome: R-HSA-9710421
Reactome: R-HSA-977225
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Histone H2ax antibody
Anti-H2a/X antibody
Anti-Histone H2a.x antibody
Anti-H2AX antibody
Anti-H2AFX antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance