• Western blot analysis of A549 cells using Acetyl-EF-1 Alpha 1/2 (K41) Polyclonal Antibody. Secondary antibody was diluted at 1:20000
  • Western blot analysis of lysate from A549 cells, using EEF1A-pan (Acetyl-Lys41) Antibody.
  • Western blot analysis of A549 cells using Acetyl-EF-1 Alpha 1/2 (K41) Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-Acetyl-EEF1A1-Lys41 antibody (1-50) (STJ90143)


Current Stock:
Host: Rabbit
Applications: WB, ELISA
Reactivity: Human, Mouse, Rat
Short Description: Rabbit polyclonal antibody anti-Acetyl-Elongation Factor 1-Alpha 1-Lys41 (1-50) is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS, 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit anti-serum by affinity-chromatography.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EEF1A1
Gene ID: 1915
Uniprot ID: EF1A1_HUMAN
Immunogen Region: 1-50
Specificity: Acetyl-EEF1A1-Lys41 polyclonal antibody (Elongation Factor 1-Alpha 1) binds to endogenous Elongation Factor 1-Alpha 1 at the amino acid region 1-50.
Immunogen: The antiserum was produced against synthesized Acetyl-peptide derived from human EEF1A around the Acetylation site of Lys41 at amino acid range 1-50
Post Translational Modifications ISGylated. Phosphorylated by TXK. Phosphorylation by PASK increases translation efficiency. Phosphorylated by ROCK2. Trimethylated at Lys-79 by EEF1AKMT1. Methylated at Lys-165 by EEF1AKMT3, methylation by EEF1AKMT3 is dynamic as well as inducible by stress conditions, such as ER-stress, and plays a regulatory role on mRNA translation. Trimethylated at Lys-318 by EEF1AKMT2. Mono-, di-, and trimethylated at Lys-36 by EEF1AKMT4.trimethylated form is predominant. Methylation by EEF1AKMT4 contributes to the fine-tuning of translation rates for a subset of tRNAs. Trimethylated at Gly-2 by METTL13. Mono- and dimethylated at Lys-55 by METTL13.dimethylated form is predominant.
Function This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Plays a role in the positive regulation of IFNG transcription in T-helper 1 cells as part of an IFNG promoter-binding complex with TXK and PARP1. (Microbial infection) Required for the translation of viral proteins and viral replication during human coronavirus SARS-CoV-2 infection.
Protein Name Elongation Factor 1-Alpha 1
Elongation Factor Tu
Eukaryotic Elongation Factor 1 A-1
Leukocyte Receptor Cluster Member 7
Database Links Reactome: R-HSA-156842
Reactome: R-HSA-156902
Reactome: R-HSA-3371511
Reactome: R-HSA-6798695
Reactome: R-HSA-8876725
Reactome: R-HSA-9613829
Cellular Localisation Cytoplasm
Cell Membrane
Colocalizes With Dlc1 At Actin-Rich Regions In The Cell Periphery
Translocates Together With Zpr1 From The Cytoplasm To The Nucleus And Nucleolus After Treatment With Mitogens
Localization At The Cell Membrane Depends On Eef1a1 Phosphorylation Status And The Presence Of Ppp1r16b
Alternative Antibody Names Anti-Elongation Factor 1-Alpha 1 antibody
Anti-Ef-1-Alpha-1 antibody
Anti-Elongation Factor Tu antibody
Anti-Ef-Tu antibody
Anti-Eukaryotic Elongation Factor 1 A-1 antibody
Anti-Eef1a-1 antibody
Anti-Leukocyte Receptor Cluster Member 7 antibody
Anti-EEF1A1 antibody
Anti-EEF1A antibody
Anti-EF1A antibody
Anti-LENG7 antibody

Information sourced from Uniprot.org

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