Anti-Acetyl-EEF1A1-Lys41 antibody (1-50 aa) (STJ90143)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized Acetyl-peptide derived from the human EEF1A around the Acetylation site of Lys41 at the amino acid range 1-50
General Information
| Short Description | Rabbit polyclonal anti-Acetyl-Elongation factor 1-alpha 1-Lys41 (1-50 aa) for use in WB and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000ELISA 1:20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | EEF1A1 |
| Gene ID | 1915 |
| Uniprot ID | EF1A1_HUMAN |
| Immunogen | The antiserum was produced against synthesized Acetyl-peptide derived from the human EEF1A around the Acetylation site of Lys41 at the amino acid range 1-50 |
| Immunogen Region | 1-50 aa |
| Specificity | Acetyl-EF-1 Alpha 1/2 (K41) Polyclonal Antibody detects endogenous levels of EF-1 Alpha 1/2 protein only when acetylated at K41. |
Additional Info
| Function | Translation elongation factor that catalyzes the GTP-dependent binding of aminoacyl-tRNA (aa-tRNA) to the A-site of ribosomes during the elongation phase of protein synthesis. Base pairing between the mRNA codon and the aa-tRNA anticodon promotes GTP hydrolysis, releasing the aa-tRNA from EEF1A1 and allowing its accommodation into the ribosome. The growing protein chain is subsequently transferred from the P-site peptidyl tRNA to the A-site aa-tRNA, extending it by one amino acid through ribosome-catalyzed peptide bond formation. Also plays a role in the positive regulation of IFNG transcription in T-helper 1 cells as part of an IFNG promoter-binding complex with TXK and PARP1. Also plays a role in cytoskeleton organization by promoting actin bundling. (Microbial infection) Required for the translation of viral proteins and viral replication during human coronavirus SARS-CoV-2 infection. |
| Protein Name | Elongation Factor 1-Alpha 1Ef-1-Alpha-1Elongation Factor TuEf-TuEukaryotic Elongation Factor 1 A-1Eef1a-1Leukocyte Receptor Cluster Member 7 |
| Database Links | Reactome: R-HSA-156842Reactome: R-HSA-156902Reactome: R-HSA-3371511Reactome: R-HSA-6798695Reactome: R-HSA-8876725Reactome: R-HSA-9613829Reactome: R-HSA-9735869 |
| Cellular Localisation | CytoplasmNucleusNucleolusCell MembraneColocalizes With Dlc1 At Actin-Rich Regions In The Cell PeripheryTranslocates Together With Zpr1 From The Cytoplasm To The Nucleus And Nucleolus After Treatment With MitogensLocalization At The Cell Membrane Depends On Eef1a1 Phosphorylation Status And The Presence Of Ppp1r16b |
| Alternative Antibody Names | Anti-Elongation Factor 1-Alpha 1 antibodyAnti-Ef-1-Alpha-1 antibodyAnti-Elongation Factor Tu antibodyAnti-Ef-Tu antibodyAnti-Eukaryotic Elongation Factor 1 A-1 antibodyAnti-Eef1a-1 antibodyAnti-Leukocyte Receptor Cluster Member 7 antibodyAnti-EEF1A1 antibodyAnti-EEF1A antibodyAnti-EF1A antibodyAnti-LENG7 antibody |
Information sourced from Uniprot.org