• Western blot analysis of JK cells using Acetyl-Ref-1 (K7) Polyclonal Antibody. Secondary antibody was diluted at 1:20000
  • Western blot analysis of lysate from Jurkat cells, using APE1 (Acetyl-Lys7) Antibody.
  • Western blot analysis of Jurkat, K562 cells using Acetyl-Ref-1 (K7) Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-Acetyl-APEX1-Lys7 antibody (1-50) (STJ90138)

SKU:
STJ90138

Current Stock:
Host: Rabbit
Applications: WB, ELISA
Reactivity: Human, Rat, Mouse
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit polyclonal antibody anti-Acetyl-Dna (Apurinic Or Apyrimidinic Site Endonuclease-Lys7 (1-50) is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS, 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit anti-serum by affinity-chromatography.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: APEX1
Gene ID: 328
Uniprot ID: APEX1_HUMAN
Immunogen Region: 1-50
Specificity: Acetyl-APEX1-Lys7 polyclonal antibody (Dna- (Apurinic Or Apyrimidinic Site Endonuclease) binds to endogenous Dna- (Apurinic Or Apyrimidinic Site Endonuclease at the amino acid region 1-50.
Immunogen: The antiserum was produced against synthesized Acetyl-peptide derived from human APE1 around the Acetylation site of Lys7 at amino acid range 1-50
Function Multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 are DNA repair and redox regulation of transcriptional factors. Functions as a apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Does also incise at AP sites in the DNA strand of DNA/RNA hybrids, single-stranded DNA regions of R-loop structures, and single-stranded RNA molecules. Has a 3'-5' exoribonuclease activity on mismatched deoxyribonucleotides at the 3' termini of nicked or gapped DNA molecules during short-patch BER. Possesses a DNA 3' phosphodiesterase activity capable of removing lesions (such as phosphoglycolate) blocking the 3' side of DNA strand breaks. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation. Acts as a loading factor for POLB onto non-incised AP sites in DNA and stimulates the 5'-terminal deoxyribose 5'-phosphate (dRp) excision activity of POLB. Plays a role in the protection from granzymes-mediated cellular repair leading to cell death. Also involved in the DNA cleavage step of class switch recombination (CSR). On the other hand, APEX1 also exerts reversible nuclear redox activity to regulate DNA binding affinity and transcriptional activity of transcriptional factors by controlling the redox status of their DNA-binding domain, such as the FOS/JUN AP-1 complex after exposure to IR. Involved in calcium-dependent down-regulation of parathyroid hormone (PTH) expression by binding to negative calcium response elements (nCaREs). Together with HNRNPL or the dimer XRCC5/XRCC6, associates with nCaRE, acting as an activator of transcriptional repression. Stimulates the YBX1-mediated MDR1 promoter activity, when acetylated at Lys-6 and Lys-7, leading to drug resistance. Acts also as an endoribonuclease involved in the control of single-stranded RNA metabolism. Plays a role in regulating MYC mRNA turnover by preferentially cleaving in between UA and CA dinucleotides of the MYC coding region determinant (CRD). In association with NMD1, plays a role in the rRNA quality control process during cell cycle progression. Associates, together with YBX1, on the MDR1 promoter. Together with NPM1, associates with rRNA. Binds DNA and RNA.
Protein Name Dna-(Apurinic Or Apyrimidinic Site Endonuclease
Apex Nuclease
Apen
Apurinic-Apyrimidinic Endonuclease 1
Ap Endonuclease 1
Ape-1
Ref-1
Redox Factor-1 Cleaved Into - Dna-(Apurinic Or Apyrimidinic Site Endonuclease - Mitochondrial
Database Links Reactome: R-HSA-110357
Reactome: R-HSA-110362
Reactome: R-HSA-110373
Reactome: R-HSA-5651801
Reactome: R-HSA-73930
Reactome: R-HSA-73933
Cellular Localisation Nucleus
Nucleolus
Nucleus Speckle
Endoplasmic Reticulum
Cytoplasm
Detected In The Cytoplasm Of B-Cells Stimulated To Switch
Colocalized With Sirt1 In The Nucleus
Colocalized With Ybx1 In Nuclear Speckles After Genotoxic Stress
Together With Ogg1 Is Recruited To Nuclear Speckles In Uva-Irradiated Cells
Colocalized With Nucleolin And Npm1 In The Nucleolus
Its Nucleolar Localization Is Cell Cycle Dependent And Requires Active Rrna Transcription
Colocalized With Calreticulin In The Endoplasmic Reticulum
Translocation From The Nucleus To The Cytoplasm Is Stimulated In Presence Of Nitric Oxide (No) And Function In A Crm1-Dependent Manner
Possibly As A Consequence Of Demasking A Nuclear Export Signal (Amino Acid Position 64-80)
S-Nitrosylation At Cys-93 And Cys-310 Regulates Its Nuclear-Cytosolic Shuttling
Ubiquitinated Form Is Localized Predominantly In The Cytoplasm
Dna-(Apurinic Or Apyrimidinic Site) Endonuclease
Mitochondrial: Mitochondrion
The Cleaved Apex2 Is Only Detected In Mitochondria
Translocation From The Cytoplasm To The Mitochondria Is Mediated By Ros Signaling And Cleavage Mediated By Granzyme A
Tom20-Dependent Translocated Mitochondrial Apex1 Level Is Significantly Increased After Genotoxic Stress
Alternative Antibody Names Anti-Dna-(Apurinic Or Apyrimidinic Site Endonuclease antibody
Anti-Apex Nuclease antibody
Anti-Apen antibody
Anti-Apurinic-Apyrimidinic Endonuclease 1 antibody
Anti-Ap Endonuclease 1 antibody
Anti-Ape-1 antibody
Anti-Ref-1 antibody
Anti-Redox Factor-1 Cleaved Into - Dna-(Apurinic Or Apyrimidinic Site Endonuclease - Mitochondrial antibody
Anti-APEX1 antibody
Anti-APE antibody
Anti-APE1 antibody
Anti-APEX antibody
Anti-APX antibody
Anti-HAP1 antibody
Anti-REF1 antibody

Information sourced from Uniprot.org

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