• Western blot analysis of extracts of various cell lines, using ACC1 antibody (STJ118055) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using ACC1 rabbit polyclonal antibody (STJ118055) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver using ACC1 rabbit polyclonal antibody (STJ118055) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of C6 using ACC1 rabbit polyclonal antibody (STJ118055) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 using ACC1 rabbit polyclonal antibody (STJ118055) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g ACC1 antibody (STJ118055). Western blot was performed from the immunoprecipitate using ACC1 antibody (STJ118055) at a dilution of 1:1000.

Anti-Acetyl-ACACA antibody (2266-2346) (STJ118055)

SKU:
STJ118055

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Acetyl-CoA carboxylase alpha (2266-2346) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACACA
Gene ID: 31
Uniprot ID: ACACA_HUMAN
Immunogen Region: 2266-2346
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 2266-2346 of human ACC1 (NP_942133.1).
Immunogen Sequence: NNKDLAEWLEKQLTEEDGVH SVIEENIKCISRDYVLKQIR SLVQANPEVAMDSIIHMTQH ISPTQRAEVIRILSTMDSPS T
Tissue Specificity Expressed in brain, placenta, skeletal muscle, renal, pancreatic and adipose tissues.expressed at low level in pulmonary tissue.not detected in the liver.
Post Translational Modifications Phosphorylation on Ser-1263 is required for interaction with BRCA1. Phosphorylation at Ser-80 by AMPK inactivates enzyme activity. The biotin cofactor is covalently attached to the central biotinyl-binding domain and is required for the catalytic activity.
Function Cytosolic enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the first and rate-limiting step of de novo fatty acid biosynthesis. This is a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA.
Protein Name Acetyl-Coa Carboxylase 1
Acc1
Acetyl-Coenzyme A Carboxylase Alpha
Acc-Alpha
Database Links Reactome: R-HSA-163765
Reactome: R-HSA-196780
Reactome: R-HSA-200425
Reactome: R-HSA-2426168
Reactome: R-HSA-3371599
Reactome: R-HSA-75105
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Acetyl-Coa Carboxylase 1 antibody
Anti-Acc1 antibody
Anti-Acetyl-Coenzyme A Carboxylase Alpha antibody
Anti-Acc-Alpha antibody
Anti-ACACA antibody
Anti-ACAC antibody
Anti-ACC1 antibody
Anti-ACCA antibody

Information sourced from Uniprot.org

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