• Western blot analysis of mouse lung, using Aggrecan rabbit monoclonal antibody (STJ11101391) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded rat bone marrow using Aggrecan rabbit monoclonal antibody (STJ11101391) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse bone marrow using Aggrecan rabbit monoclonal antibody (STJ11101391) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-ACAN antibody (761-861) [S1MR] (STJ11101391)

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STJ11101391

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Aggrecan (761-861) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACAN
Gene ID: 176
Uniprot ID: PGCA_HUMAN
Immunogen Region: 761-861
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 761-861 of human Aggrecan (P16112).
Immunogen Sequence: WPPTGAATEESTEGPSATEV PSASEEPSPSEVPFPSEEPS PSEEPFPSVRPFPSVELFPS EEPFPSKEPSPSEEPSASEE PYTPSPPVPSWTELPSSGEE S
Tissue Specificity Detected in fibroblasts (at protein level). Restricted to cartilage.
Post Translational Modifications Contains mostly chondroitin sulfate, but also keratan sulfate chains, N-linked and O-linked oligosaccharides. The release of aggrecan fragments from articular cartilage into the synovial fluid at all stages of human osteoarthritis is the result of cleavage by aggrecanase.
Function This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.
Protein Name Aggrecan Core Protein
Cartilage-Specific Proteoglycan Core Protein
Cspcp
Chondroitin Sulfate Proteoglycan Core Protein 1
Chondroitin Sulfate Proteoglycan 1 Cleaved Into - Aggrecan Core Protein 2
Database Links Reactome: R-HSA-1474228
Reactome: R-HSA-2022854
Reactome: R-HSA-2022857
Reactome: R-HSA-3000178
Reactome: R-HSA-3656225
Reactome: R-HSA-3656243
Reactome: R-HSA-3656244
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Aggrecan Core Protein antibody
Anti-Cartilage-Specific Proteoglycan Core Protein antibody
Anti-Cspcp antibody
Anti-Chondroitin Sulfate Proteoglycan Core Protein 1 antibody
Anti-Chondroitin Sulfate Proteoglycan 1 Cleaved Into - Aggrecan Core Protein 2 antibody
Anti-ACAN antibody
Anti-AGC1 antibody
Anti-CSPG1 antibody
Anti-MSK16 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance