• Western blot analysis of extracts of various cell lines, using ACADVL antibody (STJ110175) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Immunofluorescence analysis of HeLa cells using ACADVL antibody (STJ110175) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using ACADVL antibody (STJ110175) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using ACADVL antibody (STJ110175) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-ACADVL antibody (1-140) (STJ110175)

SKU:
STJ110175

£169.50 - £449.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ACADVL (1-140) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACADVL
Gene ID: 37
Uniprot ID: ACADV_HUMAN
Immunogen Region: 1-140
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human ACADVL (NP_000009.1).
Immunogen Sequence: MQAARMAASLGRQLLRLGGG SSRLTALLGQPRPGPARRPY AGGAAQLALDKSDSHPSDAL TRKKPAKAESKSFAVGMFKG QLTTDQVFPYPSVLNEEQTQ FLKELVEPVSRFFEEVNDPA KNDALEMVEETTWQGLKELG
Tissue Specificity Predominantly expressed in heart and skeletal muscle (at protein level). Also detected in kidney and liver (at protein level).
Post Translational Modifications S-nitrosylation at Cys-237 in liver improves catalytic efficiency.
Function Very long-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats. The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA. Among the different mitochondrial acyl-CoA dehydrogenases, very long-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 12 to 24 carbons long primary chains.
Protein Name Very Long-Chain Specific Acyl-Coa Dehydrogenase - Mitochondrial
Vlcad
Database Links Reactome: R-HSA-381038
Reactome: R-HSA-77305
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Isoform 2: Mitochondrion Inner Membrane
Alternative Antibody Names Anti-Very Long-Chain Specific Acyl-Coa Dehydrogenase - Mitochondrial antibody
Anti-Vlcad antibody
Anti-ACADVL antibody
Anti-VLCAD antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance