• Western blot analysis of extracts of various cell lines, using ABHD12 rabbit polyclonal antibody (STJ11102925) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ABHD12 antibody (STJ11102925)

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human, Rat
Short Description: Rabbit polyclonal antibody anti-ABHD12 is suitable for use in Western Blot.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS containing 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store in a freezer at-20°C and avoid freeze-thaw cycles.
Gene Symbol: ABHD12
Gene ID: 26090
Uniprot ID: ABD12_HUMAN
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 110-250 of human ABHD12 (NP_056415.1).
Post Translational Modifications Glycosylated.glycosylation is required for optimal activity.
Function Lysophosphatidylserine (LPS) lipase that mediates the hydrolysis of lysophosphatidylserine, a class of signaling lipids that regulates immunological and neurological processes. Represents a major lysophosphatidylserine lipase in the brain, thereby playing a key role in the central nervous system. Also able to hydrolyze oxidized phosphatidylserine.oxidized phosphatidylserine is produced in response to severe inflammatory stress and constitutes a proapoptotic 'eat me' signal. Also has monoacylglycerol (MAG) lipase activity: hydrolyzes 2-arachidonoylglycerol (2-AG), thereby acting as a regulator of endocannabinoid signaling pathways. Has a strong preference for very-long-chain lipid substrates.substrate specificity is likely due to improved catalysis and not improved substrate binding.
Protein Name Lysophosphatidylserine Lipase Abhd12
2-Arachidonoylglycerol Hydrolase Abhd12
Abhydrolase Domain-Containing Protein 12
Monoacylglycerol Lipase Abhd12
Oxidized Phosphatidylserine Lipase Abhd12
Database Links Reactome: R-HSA-426048
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Membrane Protein
Alternative Antibody Names Anti-Lysophosphatidylserine Lipase Abhd12 antibody
Anti-2-Arachidonoylglycerol Hydrolase Abhd12 antibody
Anti-Abhydrolase Domain-Containing Protein 12 antibody
Anti-Habhd12 antibody
Anti-Monoacylglycerol Lipase Abhd12 antibody
Anti-Oxidized Phosphatidylserine Lipase Abhd12 antibody
Anti-ABHD12 antibody
Anti-C20orf22 antibody

Information sourced from Uniprot.org

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