• Western blot analysis of extracts of various cell lines, using AASS antibody (STJ114729) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-AASS antibody (180-400) (STJ114729)

SKU:
STJ114729

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AASS (180-400) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AASS
Gene ID: 10157
Uniprot ID: AASS_HUMAN
Immunogen Region: 180-400
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 180-400 of human AASS (NP_005754.2).
Immunogen Sequence: MHIGMAHNYRNSSQAVQAVR DAGYEISLGLMPKSIGPLTF VFTGTGNVSKGAQAIFNELP CEYVEPHELKEVSQTGDLRK VYGTVLSRHHHLVRKTDAVY DPAEYDKHPERYISRFNTDI APYTTCLINGIYWEQNTPRL LTRQDAQSLLAPGKFSPAGV EGCPALPHKLVAICDISADT GGSIEFMTECTTIEHPFCMY DADQHIIHDSVEGSGILMCS I
Tissue Specificity Expressed in all 16 tissues examined with highest expression in the liver.
Function Bifunctional enzyme that catalyzes the first two steps in lysine degradation.
Protein Name Alpha-Aminoadipic Semialdehyde Synthase - Mitochondrial
Lkr/Sdh Includes - Lysine Ketoglutarate Reductase
Lkr
Lor - Saccharopine Dehydrogenase
Sdh
Database Links Reactome: R-HSA-71064
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Alpha-Aminoadipic Semialdehyde Synthase - Mitochondrial antibody
Anti-Lkr/Sdh Includes - Lysine Ketoglutarate Reductase antibody
Anti-Lkr antibody
Anti-Lor - Saccharopine Dehydrogenase antibody
Anti-Sdh antibody
Anti-AASS antibody

Information sourced from Uniprot.org

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