• WB on mouse tissue lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody dilution 1: 2000 incubated overnight at 4C.
  • IHC-P on paraffin sections of mouse spleen.The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
  • IHC-P on paraffin sections of mouse spleen.The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
  • IHC-P on paraffin sections of mouse spleen.The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
  • IHC-P on paraffin sections of mouse spleen.The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.

Anti-5HT1B antibody (STJ13100351)

SKU:
STJ13100351

£302.50
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Host: NZ White Rabbit
Applications: IHC, WB
Reactivity: Mouse
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Nz White Rabbit polyclonal antibody anti-5HT1B R is suitable for use in Immunohistochemistry and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Shipped as lyophilised. Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Purification: Whole serum
Dilution Range: A dilution of 1:2000 is recommended for WB and 1:1000 for IHC-P. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Storage Instruction: Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Gene Symbol: HTR1B
Gene ID: 3351
Uniprot ID: 5HT1B_HUMAN
Specificity: Specific for 5HT1B receptor.
Immunogen: A synthetic peptide from mouse 5HT1B receptor conjugated to blue carrier protein was used as the antigen.
Post Translational Modifications Phosphorylated. Desensitization of the receptor may be mediated by its phosphorylation. Palmitoylated.
Function G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for ergot alkaloid derivatives, various anxiolytic and antidepressant drugs and other psychoactive substances, such as lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity. Arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Regulates the release of 5-hydroxytryptamine, dopamine and acetylcholine in the brain, and thereby affects neural activity, nociceptive processing, pain perception, mood and behavior. Besides, plays a role in vasoconstriction of cerebral arteries.
Protein Name 5-Hydroxytryptamine Receptor 1b
5-Ht-1b
5-Ht1b
S12
Serotonin 1d Beta Receptor
5-Ht-1d-Beta
Serotonin Receptor 1b
Database Links Reactome: R-HSA-390666
Reactome: R-HSA-418594
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-5-Hydroxytryptamine Receptor 1b antibody
Anti-5-Ht-1b antibody
Anti-5-Ht1b antibody
Anti-S12 antibody
Anti-Serotonin 1d Beta Receptor antibody
Anti-5-Ht-1d-Beta antibody
Anti-Serotonin Receptor 1b antibody
Anti-HTR1B antibody
Anti-HTR1DB antibody

Information sourced from Uniprot.org

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