• Western blot analysis of lysates from Jurkat and HUVEC cells, using 53BP1 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-53BP1 antibody (STJ91390)

SKU:
STJ91390

Current Stock:
Host: Rabbit
Applications: WB, ELISA
Reactivity: Human, Mouse
Short Description: Rabbit polyclonal anti-53BP1 antibody is suitable for use in Western Blot and ELISA.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG
Dilution Range: WB 1:500-1:2000
ELISA 1:10000
Specificity: 53BP1 polyclonal antibody detects endogenous levels of 53BP1 protein.
Uniprot ID: TP53B_HUMAN
Gene ID: 7158
Gene Symbol: TP53BP1
Immunogen: Synthesized peptide derived from human 53BP1 around the non-phosphorylation site of S29.
Immunogen Region: 10-90 aa
Function Double-strand break (DSB) repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR) during antibody genesis. Plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1. In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites. Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites. Required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs. Participates in the repair and the orientation of the broken DNA ends during CSR. In contrast, it is not required for classic NHEJ and V(D)J recombination. Promotes NHEJ of dysfunctional telomeres via interaction with PAXIP1.
Protein Name Tp53-Binding Protein 1
53bp1
P53-Binding Protein 1
P53bp1
Database Links Reactome: R-HSA-3232118
Reactome: R-HSA-5693565
Reactome: R-HSA-5693571
Reactome: R-HSA-5693607
Reactome: R-HSA-69473
Cellular Localisation Nucleus
Chromosome
Centromere
Kinetochore
Localizes To The Nucleus In Absence Of Dna Damage
Following Dna Damage
Recruited To Sites Of Dna Damage
Such As Double Stand Breaks (Dsbs): Recognizes And Binds Histone H2a Monoubiquitinated At 'Lys-15' (H2ak15ub) And Histone H4 Dimethylated At 'Lys-20' (H4k20me2)
Two Histone Marks That Are Present At Dsbs Sites
Associated With Kinetochores During Mitosis
Alternative Antibody Names Anti-Tp53-Binding Protein 1 antibody
Anti-53bp1 antibody
Anti-P53-Binding Protein 1 antibody
Anti-P53bp1 antibody
Anti-TP53BP1 antibody

Information sourced from Uniprot.org

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