• STJ70487 staining (0.25µg/ml) of 293 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • STJ70487 staining (0.25µg/ml) of 293 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Anti-4E-T/EIF4ENIF1 Antibody (STJ70487)

SKU:
STJ70487

Current Stock:
Host: Goat
Applications: Pep-ELISA, WB, IHC
Reactivity: Human, Mouse, Rat, Dog, Pig, Bovine
Short Description: Goat polyclonal anti-4E-T/EIF4ENIF1 antibody is suitable for use in ELISA, Western Blot and Immunohistochemistry.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at-20°C. Minimize freezing and thawing.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage Instruction: Store at-20°C and avoid freeze-thaw cycles.
Concentration: 0.5mg/mL
Isotype: IgG
Dilution Range: WB-Recommended for use at 0.25-0.5µg/ml
IHC-Recommended concentration, 5-10µg/ml
ELISA-antibody detection limit dilution 1:32000.
Specificity: This antibody is expected to recognise isoform 1 (NP_062817.2) and isoform b (NP_001157974.1). Reported variants represent identical protein (NP_062817.2; NP_001157973.1).
Uniprot ID: 4ET_MOUSE
Gene ID: 74203
Gene Symbol: Eif4enif1
Immunogen Region: C Terminus
Immunogen Sequence: AKVISVDELEYRQ
Accession Number: NP_062817.2; NP_001157974.1
Post Translational Modifications Phosphorylation by MAPK8/JNK1 and or MAPK9/JNK2 in response to oxidative stress promotes P-body assembly. Phosphorylated during meiotic maturation.
Function EIF4E-binding protein that regulates translation and stability of mRNAs in processing bodies (P-bodies). Plays a key role in P-bodies to coordinate the storage of translationally inactive mRNAs in the cytoplasm and prevent their degradation. Acts as a binding platform for multiple RNA-binding proteins: promotes deadenylation of mRNAs via its interaction with the CCR4-NOT complex, and blocks decapping via interaction with eIF4E (EIF4E and EIF4E2), thereby protecting deadenylated and repressed mRNAs from degradation. Component of a multiprotein complex that sequesters and represses translation of proneurogenic factors during neurogenesis. Promotes miRNA-mediated translational repression. Involved in mRNA translational repression mediated by the miRNA effector TNRC6B by protecting TNRC6B-targeted mRNAs from decapping and subsequent decay. Required for the formation of P-bodies. Also acts as a nucleoplasmic shuttling protein, which mediates the nuclear import of EIF4E and DDX6 by a piggy-back mechanism.
Protein Name Eukaryotic Translation Initiation Factor 4e Transporter
4e-T
Eif4e Transporter
Cd40 Ligand-Activated Specific Transcript 4
Eukaryotic Translation Initiation Factor 4e Nuclear Import Factor 1
Cellular Localisation Cytoplasm
Nucleus
Pml Body
Nucleus Speckle
Predominantly Cytoplasmic
Mainly Localizes To Processing Bodies (P-Bodies)
Shuttles Between The Nucleus And The Cytoplasm In A Crm1-Dependent Manner
Localization To Nuclear Foci And Speckles Requires Active Transcription
Alternative Antibody Names Anti-Eukaryotic Translation Initiation Factor 4e Transporter antibody
Anti-4e-T antibody
Anti-Eif4e Transporter antibody
Anti-Cd40 Ligand-Activated Specific Transcript 4 antibody
Anti-Eukaryotic Translation Initiation Factor 4e Nuclear Import Factor 1 antibody
Anti-Eif4enif1 antibody
Anti-Clast4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance