||Pep-ELISA, WB, IHC
||Human, Mouse, Rat, Dog, Pig, Bovine
||Goat polyclonal anti-4E-T/EIF4ENIF1 antibody is suitable for use in ELISA, Western Blot and Immunohistochemistry.
||FOR RESEARCH USE ONLY (RUO).
||Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at-20°C. Minimize freezing and thawing.
||Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
||Store at-20°C and avoid freeze-thaw cycles.
||WB-Recommended for use at 0.25-0.5µg/mlIHC-Recommended concentration, 5-10µg/mlELISA-antibody detection limit dilution 1:32000.
||This antibody is expected to recognise isoform 1 (NP_062817.2) and isoform b (NP_001157974.1). Reported variants represent identical protein (NP_062817.2; NP_001157973.1).
| Post Translational Modifications || Phosphorylation by MAPK8/JNK1 and or MAPK9/JNK2 in response to oxidative stress promotes P-body assembly. Phosphorylated during meiotic maturation. |
| Function || EIF4E-binding protein that regulates translation and stability of mRNAs in processing bodies (P-bodies). Plays a key role in P-bodies to coordinate the storage of translationally inactive mRNAs in the cytoplasm and prevent their degradation. Acts as a binding platform for multiple RNA-binding proteins: promotes deadenylation of mRNAs via its interaction with the CCR4-NOT complex, and blocks decapping via interaction with eIF4E (EIF4E and EIF4E2), thereby protecting deadenylated and repressed mRNAs from degradation. Component of a multiprotein complex that sequesters and represses translation of proneurogenic factors during neurogenesis. Promotes miRNA-mediated translational repression. Involved in mRNA translational repression mediated by the miRNA effector TNRC6B by protecting TNRC6B-targeted mRNAs from decapping and subsequent decay. Required for the formation of P-bodies. Also acts as a nucleoplasmic shuttling protein, which mediates the nuclear import of EIF4E and DDX6 by a piggy-back mechanism. |
| Protein Name || Eukaryotic Translation Initiation Factor 4e Transporter4e-TEif4e TransporterCd40 Ligand-Activated Specific Transcript 4Eukaryotic Translation Initiation Factor 4e Nuclear Import Factor 1 |
| Cellular Localisation || CytoplasmNucleusPml BodyNucleus SpecklePredominantly CytoplasmicMainly Localizes To Processing Bodies (P-Bodies)Shuttles Between The Nucleus And The Cytoplasm In A Crm1-Dependent MannerLocalization To Nuclear Foci And Speckles Requires Active Transcription |
| Alternative Antibody Names || Anti-Eukaryotic Translation Initiation Factor 4e Transporter antibodyAnti-4e-T antibodyAnti-Eif4e Transporter antibodyAnti-Cd40 Ligand-Activated Specific Transcript 4 antibodyAnti-Eukaryotic Translation Initiation Factor 4e Nuclear Import Factor 1 antibodyAnti-Eif4enif1 antibodyAnti-Clast4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance