• Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-4E-BP1 (phospho-Thr37/46) antibody (STJ194699)

SKU:
STJ194699

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human, Mouse, Rat
Short Description: Rabbit polyclonal anti-4E-BP1 (phospho-Thr37/46) antibody is suitable for use in Western Blot.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using a specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG
Dilution Range: WB 1:1000-2000
Specificity: This antibody detects endogenous levels of Human Mouse Rat 4E-BP1 (phospho-Thr37 or 46)
Uniprot ID: 4EBP1_HUMAN
Gene ID: 1978
Gene Symbol: EIF4EBP1
Immunogen: Synthesized phosho peptide around human 4E-BP1 (Thr37 and 46)
Function Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
Protein Name Eukaryotic Translation Initiation Factor 4e-Binding Protein 1
4e-Bp1
Eif4e-Binding Protein 1
Phosphorylated Heat- And Acid-Stable Protein Regulated By Insulin 1
Phas-I
Database Links Reactome: R-HSA-166208
Reactome: R-HSA-72662
Alternative Antibody Names Anti-Eukaryotic Translation Initiation Factor 4e-Binding Protein 1 antibody
Anti-4e-Bp1 antibody
Anti-Eif4e-Binding Protein 1 antibody
Anti-Phosphorylated Heat- And Acid-Stable Protein Regulated By Insulin 1 antibody
Anti-Phas-I antibody
Anti-EIF4EBP1 antibody

Information sourced from Uniprot.org

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