• Immunohistochemistry analysis of paraffin-embedded human pancreas carcinoma (A) , esophagus carcinoma tissue (B) and ovary tumor tissue (C) , showing cytoplasmic and membrane localization with DAB staining using 4E-BP1 monoclonal antibody.
  • Western blot analysis using 4E-BP1 monoclonal antibody against truncated 4E-BP1 recombinant protein (1) and A431 cell lysate (2).

Anti-4E-BP1 antibody (STJ97804)

Current Stock:
Host: Mouse
Applications: WB, IHC-P, ELISA
Reactivity: Human
Short Description: Mouse monoclonal primary antibody against 4E-BP1.
Clonality: Monoclonal
Clone ID: 4B6G10
Conjugation: Unconjugated
Formulation: Ascitic fluid containing 0.03% sodium azide, 0.5% BSA, 50% glycerol.
Purification: Affinity purification
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/mL
Isotype: IgG1
Dilution Range: WB 1:500-1:2000
IHC 1:200-1:1000
ELISA 1:10000
Specificity: 4E-BP1 monoclonal antibody detects endogenous levels of 4E-BP1 protein.
Uniprot ID: 4EBP1_HUMAN
Gene ID: 1978
Gene Symbol: EIF4EBP1
Immunogen: Purified recombinant fragment of 4E-BP1 expressed in E. Coli.
Post Translational Modifications Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70, corresponding to the hyperphosphorylated form, is regulated by mTORC1 and abolishes binding to EIF4E. Ubiquitinated: when eIF4E levels are low, hypophosphorylated form is ubiquitinated by the BCR(KLHL25) complex, leading to its degradation and serving as a homeostatic mechanism to maintain translation and prevent eIF4E inhibition when eIF4E levels are low. Not ubiquitinated when hyperphosphorylated (at Thr-37, Thr-46, Ser-65 and Thr-70) or associated with eIF4E.
Function Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
Database Links Reactome: R-HSA-166208
Protein Name Eukaryotic Translation Initiation Factor 4e-Binding Protein 1
Eif4e-Binding Protein 1
Phosphorylated Heat- And Acid-Stable Protein Regulated By Insulin 1
Alternative Names Anti-Eukaryotic Translation Initiation Factor 4e-Binding Protein 1 antibody
Anti-4e-Bp1 antibody
Anti-Eif4e-Binding Protein 1 antibody
Anti-Phosphorylated Heat- And Acid-Stable Protein Regulated By Insulin 1 antibody
Anti-Phas-I antibody
Anti-EIF4EBP1 antibody

Information sourced from Uniprot.org

6 months standard