• Western blot analysis of lysates from COS7 cells, treated with EGF 200ng/ml 30', using 4E-BP1 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Immunohistochemistry analysis of paraffin-embedded human brain tissue, using 4E-BP1 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using 4E-BP1 Polyclonal Antibody diluted at 1:500

Anti-4E-BP1 antibody (STJ91385)

SKU:
STJ91385

Current Stock:
Host: Rabbit
Applications: WB, IHC-P, ELISA
Reactivity: Mouse, Rat, Monkey
Short Description: Rabbit polyclonal anti-4E-BP1 antibody is suitable for use in Western Blot, Immunohistochemistry and ELISA.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
Specificity: 4E-BP1 polyclonal antibody detects endogenous levels of 4E-BP1 protein.
Uniprot ID: 4EBP1_HUMAN
Gene ID: 1978
Gene Symbol: EIF4EBP1
Immunogen: Synthesized peptide derived from mouse 4E-BP1 around the non-phosphorylation site of T69.
Immunogen Region: 10-90 aa
Function Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
Protein Name Eukaryotic Translation Initiation Factor 4e-Binding Protein 1
4e-Bp1
Eif4e-Binding Protein 1
Phosphorylated Heat- And Acid-Stable Protein Regulated By Insulin 1
Phas-I
Database Links Reactome: R-HSA-166208
Reactome: R-HSA-72662
Alternative Antibody Names Anti-Eukaryotic Translation Initiation Factor 4e-Binding Protein 1 antibody
Anti-4e-Bp1 antibody
Anti-Eif4e-Binding Protein 1 antibody
Anti-Phosphorylated Heat- And Acid-Stable Protein Regulated By Insulin 1 antibody
Anti-Phas-I antibody
Anti-EIF4EBP1 antibody

Information sourced from Uniprot.org

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