• Western blot analysis of lysates from COS7 cells, treated with EGF 200ng/ml 30', using 4E-BP1 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Immunohistochemistry analysis of paraffin-embedded human brain tissue, using 4E-BP1 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using 4E-BP1 Polyclonal Antibody diluted at 1:500

Anti-4E-BP1 antibody (10-90) (STJ91385)

SKU:
STJ91385

Current Stock:
Host: Rabbit
Applications: WB, IHC-P, IF-P, ELISA
Reactivity: Mouse, Rat, Monkey
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit polyclonal antibody anti-EIF4EBP1 (10-90) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS, 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit anti-serum by affinity-chromatography.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 10-90
Specificity: 4E-BP1 polyclonal antibody (EIF4EBP1) binds to endogenous EIF4EBP1 at the amino acid region 10-90.
Immunogen: The antiserum was produced against synthesized peptide derived from mouse 4E-BP1 at amino acid range 35-84
Function Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
Protein Name Eukaryotic Translation Initiation Factor 4e-Binding Protein 1
4e-Bp1
Eif4e-Binding Protein 1
Phosphorylated Heat- And Acid-Stable Protein Regulated By Insulin 1
Phas-I
Database Links Reactome: R-HSA-166208
Reactome: R-HSA-72662
Alternative Antibody Names Anti-Eukaryotic Translation Initiation Factor 4e-Binding Protein 1 antibody
Anti-4e-Bp1 antibody
Anti-Eif4e-Binding Protein 1 antibody
Anti-Phosphorylated Heat- And Acid-Stable Protein Regulated By Insulin 1 antibody
Anti-Phas-I antibody
Anti-EIF4EBP1 antibody

Information sourced from Uniprot.org

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