• Western blot analysis of extracts of various cell lines, using 14-3-3 Theta rabbit monoclonal antibody (STJ11102399) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunofluorescence analysis of NIH-3T3 cells using 14-3-3 Theta Rabbit monoclonal antibody (STJ11102399) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using 14-3-3 Theta rabbit monoclonal antibody (STJ11102399) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using 14-3-3 Theta Rabbit monoclonal antibody (STJ11102399) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-YWHAQ antibody (1-100) [S9MR] (STJ11102399)

SKU:
STJ11102399

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-14-3-3 Theta (1-100) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: YWHAQ
Gene ID: 10971
Uniprot ID: 1433T_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human 14-3-3 Theta (P27348).
Immunogen Sequence: MEKTELIQKAKLAEQAERYD DMATCMKAVTEQGAELSNEE RNLLSVAYKNVVGGRRSAWR VISSIEQKTDTSDKKLQLIK DYREKVESELRSICTTVLEL
Tissue Specificity Abundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in individuals with predominant lower motor neuron involvement.
Post Translational Modifications Ser-232 is probably phosphorylated by CK1.
Function Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.
Protein Name 14-3-3 Protein Theta
14-3-3 Protein T-Cell
14-3-3 Protein Tau
Protein Hs1
Database Links Reactome: R-HSA-111447
Reactome: R-HSA-1445148
Reactome: R-HSA-5625740
Reactome: R-HSA-5628897
Reactome: R-HSA-75035
Reactome: R-HSA-9614399
Reactome: R-HSA-9735871
Reactome: R-HSA-9755779
Cellular Localisation Cytoplasm
In Neurons
Axonally Transported To The Nerve Terminals
Alternative Antibody Names Anti-14-3-3 Protein Theta antibody
Anti-14-3-3 Protein T-Cell antibody
Anti-14-3-3 Protein Tau antibody
Anti-Protein Hs1 antibody
Anti-YWHAQ antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance